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  T CD4 + Lymphocytes


  • 1997 Revised Guidelines for Performing CD4+ T-Cell Determinations in Persons Infected with Human Immunodeficiency Virus (HIV). CDC
    MMWR January 10, 1997 Vol. 46 No. RR-2
  • Clinical applications of flow cytometry: quality assurance and immunophenotyping of peripheral blood lymphocytes. NCCLS
    NCCLS Document H42T, vol 9, no. 13 1992
  • Guidelines for flow cytometric immunophenotyping: A report from the National Institute of Allergy and Infectious Diseases, Division of AIDS.
    Theresa Calvelli, Thomas N. Denny, Helene Paxton, Rebecca Gelman, and Jonathan Kagan
    Cytometry 14:702-715, Wiley-Liss 1993  (Fichero PDF)
  • Three-Color Supplement to the NIAID DAIDS Guideline for Flow Cytometric Immunophenotyping
    Janet Nicholson, Pamela Kidd, Francis Mandy, Daniella Livnat, and Jonathan Kagan
    Cytometry (Communications in Clinical Cytometry) 26:227-230, Wiley-Liss 1996 (Fichero PDF)
  • Guidelines for the enumeration of CD4+ T lymphocytes in immunosuppressed individuals.
    CD4+ T lymphocyte Working Party. D. Barnett, G. Bird
    Clin. Lab. Haem. 1997, 19, 231-241


  Cellular Cycle


  • Guidelines for implementation of clinical DNA cytometry.
    T. Shankey, P. Rabinovitch et al.
    Cytometry 14: 472-477 1993
  • DNA cytometry consensus conference (Special Reports)
    David W. Hedley, T. Vincent Shankey, Leon L. Wheeless
    Cytometry 14:471-500 1993
  • Comparision of five cell cycle analysis models applied to 1414 flow cytometric DNA histograms of fresh frozen breast cancer
    Elisabeth Bergers, Paul J. Van Diest, and Jan P.A. Baak
    Cytometry (Communications in Clinical Cytometry) 30:54-60 1997
  • Relevance of forward scatter and side scatter in aneuploidy detection by flow cytometry
    Jordi Pétriz, Dolors Tugues, Joan García López
    Analytical Cellular Pathology 10 (1996) 243-252


  Leukemia and lymphoma immunophenotyping
  • Clinical applications of flow cytometry:  immunophenotyping of leukemic cells; proposed guideline.
    NCCLS Document H43-P, vol 13, no. 23 1993
  • U.S.-Canadian consensus recommendations on the immunophenotyppic analysis of hematologic neoplasia by flow cytometry. (Special Report)
    Raul C. Braylan, Michael J. Borowitz, Bruce H. Davis, Gregory T. Stelzer, Carleton C. Stewart
    Cytometry (Communications in Clinical Cytometry) 30:213-263 1997
  • Consensus Document on Leukemia Immunophenotyping (texto completo)
    European Working Group on Clinical Cell Analysis
    Leukemia 10, 77-895 (1996)
  • Quantitative analysis of leukocyte membrane antigen expression: normal adult values
    Arsène Bikoue, Françoise George
    Cytometry (Comunications in Clinical Cytometry) 26:137-147 1996
  • Recent Advances in Flow Cytometry: Application to the Diagnosis of Hematologic Malignancy
    C. Darrell Jennings and Kenneth A. Foon
    Blood 1997 90: 2863-2892.


  Platelet autoantibodies


  • Autoantibodies against platelet glycoproteins in autoimmune thrombocytopenic purpura: their clinical significance and response to treatment
    P Berchtold and M Wenger
    Blood Vol 81, no.5, pp. 1246-1250, 1993
  • Detection of platelet-associated antibodies by flow cytometry in hematological autoimmune disorders.
    Tazzari PL, Ricci F, Vianelli N, Tassi C, Belletti D, Pierri I, Gugliotta L, Gobbi M, Conte R
    Ann Hematol 1995 May;70(5):267-272 (PubMed)
  • Flow cytometric analysis of anti-platelet antibodies in idiopathic thrombocytopenic purpura.
    Latorraca A, Lanza F, Moretti S, Ferrari L, Reverberi R, Galluccio L, Castoldi G
    Haematologica 1994 May;79(3):269-272 (PubMed)
  • Autoimmune thrombocytopenic purpura (AITP) and acquired thrombasthenia due to autoantibodies to GP IIb-IIIa in a patient with an unusual platelet membrane glycoprotein composition.
    Macchi L, Nurden P, Marit G, Bihour C, Clofent-Sanchez G, Combrie R, Nurden AT
    Am J Hematol 1998 Feb;57(2):164-175 (PubMed)


  Paroxismal Nocturnal Hemoglobinuria


  • The use of monoclonal antibodies and flow cytometry in the diagnosis of paroxysmal nocturnal hemoglobinuria
    Sharon E. Hall, Wendell F. Rosse
    Blood, vol 87,no 12,1996;5332-5340
  • Flow Cytometric Analysis of Glycosylphosphatidyl-Inositol- Anchored Proteins to Assess Paroxysmal Nocturnal Hemoglobinuria Clone Size
    Josefa Piedras and Xavier López-Karpovitch
    Cytometry (Communications in Clinical Cytometry) 42:234-238 (2000)
  • Application of Flow Cytometry to the Diagnosis of Paroxysmal Nocturnal Hemoglobinuria
    Stephen J. Richards, Andrew C. Rawstron, and Peter Hillmen
    Cytometry (Communications in Clinical Cytometry) 42:223-233 (2000)




  • Cytometry in Cell Necrobiology: Analysis of Apoptosis and Accidental Cell Death (Necrosis)
    Zbigniew Darzynkiewicz,* Gloria Juan, Xun Li, Wojciech Gorczyca, Tomoyuki Murakami, and Frank Traganos
    Cytometry 27:1-20 (1997)
  • Identification of Nuclei From Apoptotic, Necrotic, and Viable Lymphoid Cells by Using Multiparameter Flow Cytometry
    Claudia Matteucci, Sandro Grelli, Enrico De Smaele, Carla Fontana, and Antonio Mastino
    Cytometry 35:145-153 (1999)
  • Comparison of Multiple Assays for Kinetic Detection of Apoptosis in Thymocytes Exposed to Dexamethasone or Diethylstilbesterol
    K.J. Donner, K.M. Becker, B.D. Hissong, and S. Ansar Ahmed
    Cytometry 35:80-90 (1999)
  • Expression of CD10 by Human T Cells That Undergo Apoptosis Both In Vitro and In Vivo
    Giovanna Cutrona, Nicolo` Leanza, Massimo Ulivi, Giovanni Melioli, Vito L. Burgio, Giovanni Mazzarello, Giovanni Gabutti, Silvio Roncella, and Manlio Ferrarini
    Blood,Vol 94, No 9 (November 1), 1999: pp 3067-3076


  HLA B27


  • External Quality Assessment of Flow Cytometric HLA-B27 Typing
    Wilfried H.B.M. Levering, Rene van den Beemd, Jeroen G. te Marvelde, Wil A.M. van Beers, Herbert Hooijkaas, Kees Sintnicolaas, and Jan W. Gratama
    Cytometry (Communications in Clinical Cytometry) 42:95-105 (2000)
  • HLAB27 phenotyping with flow cytometry: further improvement by multiple monoclonal antibodies
    Johannes J.M.L. Hoffmann and Willy C.M. Janssen
    Clinical Chemistry 43:10 1975-1981(1997)
  • Utilization of commercial antisera and flow cytometry in HLA B27 typing
    Orr K.,Thomson GTD, Alfa M.
    Cytometry (Communications in Clinical Cytometry)1994;18:17-20
  • Comparision of monoclonal antibodies for flow cytometry analysis of HLA B27 antigen
    Ward AM, Nikaein A.
    Cytometry (Communications in Clinical Cytometry)1995;22:65-69
  • Evaluation of Four monoclonal antibodies against HLA B27 for their reliability in HLA B27 typing with flow cytometry: Comparision with the classic microlymphocytotoxic test
    J. Neumüller, D.W.M. Schwartz
    Cytometry (Communications in Clinical Cytometry)1996;26:209-215


  Stem Cells CD34+


  • Flow Cytometric Enumeration of CD34+ Hematopoietic Stem and Progenitor Cells
    European Working Group on Clinical Cell Analysis
    Cytometry (Communications in Clinical Cytometry) 34:128-142 (1998)
  • Flow cytometry counting of CD34 + cells in whole blood
    Oscar Fornas, Joan Garcia & Jordi Petriz
    Nature Medicine Vol 6 Number 7 July 2000
  • Single Platform Flow Cytometric Absolute CD34+ Cell Counts Based on the ISHAGE Guidelines
    Michael Keeney, Ian Chin-Yee, Karin Weir, Jan Popma, Rakash Nayar, and D. Robert Sutherland
    Cytometry (Communications in Clinical Cytometry) 34:61-70 (1998)
  • Comparison Between a Lyse-and-Then-Wash Method and a Lyse-Non-Wash Technique for the Enumeration of CD34+ Hematopoietic Progenitor Cells
    P. Menéndez, O. Redondo, A. Rodriguez, M.C. Lopez-Berges, G. Ercilla, A. López, A. Durán, J. Almeida, J.A. Pérez-Simón, J.F. San Miguel, J.W. Gratama, and A. Orfao
    Cytometry (Communications in Clinical Cytometry) 34:264-271 (1998)
  • Single- Versus Dual-Platform Assays for Human CD34+ Cell Enumeration
    I.L. Barbosa, M.E. Sousa, M.I. Godinho, F. Sousa, and A. Carvalhais
    Cytometry (Communications in Clinical Cytometry) 38:274-279 (1999)




  • A practical approach to multicolor flow cytometry for immunophenotyping
    Nicole Baumgarth , Mario Roederer
    Journal of Immunological Methods 243 (2000) 77-97
  • Evaluating Fluorescence Sensitivity on Flow Cytometers: An Overview
    James C.S. Wood and Robert A. Hoffman
    Cytometry 33:256-259 (1998)
  • A Flow Cytometer Designed for Fluorescence Calibration
    Howard M. Shapiro,Nancy G. Perlmutter, and Philip G. Stein
    Cytometry 33:280-287 (1998)
  • Theoretical Basis for Sampling Statistics Useful for Detecting and Isolating Rare Cells Using Flow Cytometry and Cell Sorting
    Judah I. Rosenblatt, James A. Hokanson, Scott R. McLaughlin, and James F. Leary
    Cytometry 27:233-238 (1997)
  • Enzymatic Amplification Staining for Flow Cytometric Analysis of Cell Surface Molecules
    David Kaplan and Dawn Smith
    Cytometry 40:81-85 (2000)
  • Standardizing Flow Cytometry: A Classification System of Fluorescence Standards Used for Flow Cytometry
    A. Schwartz, G. E. Marti, R. Poon, J. W. Gratama, and E. Fernández-Repollet
    Cytometry 33:106-114 (1998)
  • Multi-Platform, Multi-Site Instrumentation and Reagent Standardization
    Norman Purvis and Greg Stelzer
    Cytometry 33:156-165 (1998)
  • Terminology and Nomenclature for standardization in Quantitative Fluorescence Cytometry
    L. Omar Henderson, Gerald E. Marti, Adolfas Gaigalas, W. Harry Hannon, and Robert F. Vogt, Jr.
    Cytometry 33:97-105 (1998)
  • 8 Color, 10-Parameter Flow Cytometry to Elucidate Complex Leukocyte Heterogeneity
    Mario Roederer, Stephen De Rosa, Rachel Gerstein, Michael Anderson, Martin Bigos, Richard Stovel, Thomas Nozaki, David Parks, Leonore Herzenberg, and Leonard Herzenberg
    Cytometry 29:328-339 (1997)
Updated: 16/12/2000